重组HRP Anti-MMP14抗体[EP1264Y] (ab194242)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EP1264Y] to MMP14
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
Related conjugates and formulations
概述
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产品名称
HRP Anti-MMP14抗体[EP1264Y]
参阅全部 MMP14 一抗 -
描述
HRP兔单克隆抗体[EP1264Y] to MMP14 -
宿主
Rabbit -
偶联物
HRP -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab185123) -
阳性对照
- IHC-P: Human breast fibroadenoma tissue.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1264Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-MMP14 antibody [EP1264Y] - Low endotoxin, Azide free (ab168726)
- Alexa Fluor® 647 Anti-MMP14 antibody [EP1264Y] (ab300749)
- Alexa Fluor® 555 Anti-MMP14 antibody [EP1264Y] (ab302548)
- Alexa Fluor® 488 Anti-MMP14 antibody [EP1264Y] (ab302582)
- PE Anti-MMP14 antibody [EP1264Y] (ab303602)
- Anti-MMP14 antibody [EP1264Y] (ab51074)
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194242于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. -
组织特异性
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻译后修饰
The precursor is cleaved by a furin endopeptidase. -
细胞定位
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 4323 Human
- Entrez Gene: 17387 Mouse
- Entrez Gene: 81707 Rat
- Omim: 600754 Human
- SwissProt: P50281 Human
- SwissProt: P53690 Mouse
- SwissProt: Q10739 Rat
- Unigene: 2399 Human
see all -
别名
- Matrix metallopeptidase 14 (membrane inserted) antibody
- Matrix metalloproteinase 14 antibody
- Matrix metalloproteinase-14 antibody
see all
图片
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IHC image of MMP14 staining in a section of formalin-fixed paraffin-embedded human breast fibroadenoma tissue*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab194242 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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