The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
功能Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
组织特异性Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
序列相似性Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻译后修饰The precursor is cleaved by a furin endopeptidase.
细胞定位Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.