The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 20 µg/ml. Requires periodate-lysine-paraformaldehyde fixative for 18-24 hrs. at 4°C.
WB: Use at a concentration of 8 - 20 µg/ml. Predicted molecular weight: 66 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
组织特异性Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
序列相似性Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻译后修饰The precursor is cleaved by a furin endopeptidase.
细胞定位Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.