Anti-MMP13抗体[LIPCO IID1] (ab80734)

概述

  • 产品名称Anti-MMP13抗体[LIPCO IID1]
    参阅全部 MMP13 一抗
  • 描述
    小鼠单克隆抗体[LIPCO IID1] to MMP13
  • 特异性This antibody recognizes pro and active forms of MMP13. We have data to indicate that this antibody may not cross react with Human. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat
    不与反应: Human
  • 免疫原

    Rat MMP13 from BC-1 cells.

  • 阳性对照
    • Conditioned, serum free medium from (TPA treated) rat keratinocyte CCL-4 cells.

性能

应用

Our Abpromise guarantee covers the use of ab80734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa. Incubate for 2 hours at room temperature.
IP Use at 2 µg/mg of lysate.

靶标

  • 功能Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • 组织特异性Seems to be specific to breast carcinomas.
  • 疾病相关Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLG 3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all

Anti-MMP13 antibody [LIPCO IID1] 图像

  • Native IP using rat cells:
    Anti-MMP13 (biotin conjugated form of ab80734, containing BSA and azide) used for IP at 2 µg/mg lysate, and for subsequent WB at 1 µg/ml.
  • anti MMP13 (form of ab80734 containing BSA and azide) at 1 µg/ml + rat cells

    Predicted band size : 54 kDa
    Observed band size : 48,60 kDa (why is the actual band size different from the predicted?)

Anti-MMP13 antibody [LIPCO IID1] (ab80734)参考文献

ab80734 has not yet been referenced specifically in any publications.

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