概述

  • 产品名称Anti-MMP13抗体
    参阅全部 MMP13 一抗
  • 描述
    兔多克隆抗体to MMP13
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Rabbit, Horse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human MMP13.

    (Peptide available as ab93499.)

  • 阳性对照
    • This antibody gave a positive signal in the following human whole cell lysates: MDA-MB-231; MCF7.

性能

应用

Our Abpromise guarantee covers the use of ab84594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 54 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 21681813

靶标

  • 功能Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • 组织特异性Seems to be specific to breast carcinomas.
  • 疾病相关Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLG 3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all

Anti-MMP13 antibody 图像

  • All lanes : Anti-MMP13 antibody (ab84594) at 1 µg/ml

    Lane 1 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

Anti-MMP13 antibody (ab84594)参考文献

This product has been referenced in:
  • Stone AV  et al. Pro-inflammatory stimulation of meniscus cells increases production of matrix metalloproteinases and additional catabolic factors involved in osteoarthritis pathogenesis. Osteoarthritis Cartilage 22:264-74 (2014). WB ; Human . Read more (PubMed: 24315792) »
  • Estrada KD  et al. Smad7 regulates terminal maturation of chondrocytes in the growth plate. Dev Biol 382:375-84 (2013). IHC-P ; Mouse . Read more (PubMed: 23994637) »

See all 3 Publications for this product

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