Anti-MMP13抗体(ab39012)
Key features and details
- Rabbit polyclonal to MMP13
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, Sandwich ELISA
- Reacts with: Mouse, Rat, Dog, Human
- Isotype: IgG
概述
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产品名称
Anti-MMP13抗体
参阅全部 MMP13 一抗 -
描述
兔多克隆抗体to MMP13 -
宿主
Rabbit -
特异性
ab39012 recognizes the latent proenzyme, at 60 Kd, as well as the active form at 48 Kd, and intermediate activation forms. It does not cross react with the other MMP family members. ab39012 recognizes the Hinge region of MMP13. -
经测试应用
适用于: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt, Sandwich ELISAmore details -
种属反应性
与反应: Mouse, Rat, Dog, Human -
免疫原
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody has been peptide-affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39012于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (11) |
Use at an assay dependent concentration.
|
WB | (2) |
1/3000 - 1/6000. Predicted molecular weight: 54 kDa. 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting.
|
IHC-Fr | (3) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 24056368
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Flow Cyt |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
Sandwich ELISA |
Use a concentration of 0.5 µg/ml.
For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab77949 as Capture. |
说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/3000 - 1/6000. Predicted molecular weight: 54 kDa. 1/3000, when using colorimetric substrates such as BCIP/NBT - 1/6000, when using chemiluminescent substrates. Dilution optimised using Chromogenic detection. When used against the reduced protein identifies a band at 60 Kd. Note: The low endogenous protein levels may require concentration of samples by ultrafiltration or ammonium sulfate precipitation prior to Western blotting. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. PubMed: 24056368 |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab77949 as Capture. |
靶标
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功能
Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process. -
组织特异性
Seems to be specific to breast carcinomas. -
疾病相关
Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 403763 Dog
- Entrez Gene: 4322 Human
- Entrez Gene: 17386 Mouse
- Entrez Gene: 171052 Rat
- Omim: 600108 Human
- SwissProt: P45452 Human
- SwissProt: P33435 Mouse
- SwissProt: P23097 Rat
see all -
别名
- CLG 3 antibody
- CLG3 antibody
- Collagenase 3 antibody
see all
图片
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All lanes : Anti-MMP13 antibody (ab39012) at 1/3000 dilution
Lane 1 : cell media from human chondrosarcoma (untreated).
Lane 2 : cell media from human chondrosarcoma (IL1 beta treated).
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
The loading amount is 15 ul/lane, and the condrosarcoma media was concentrated 40x. -
Standard curve for MMP13; dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [181-15A12] to MMP13 (ab77949) at 1µg/ml and Detector Antibody Rabbit polyclonal to MMP13 - Hinge region (ab39012) at 0.5µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (428)
ab39012 被引用在 428 文献中.
- Wu C et al. Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes Dis 11:964-977 (2024). IHC-Fr ; Mouse . PubMed: 37692479
- Li Y et al. Enhancing cartilage repair with optimized supramolecular hydrogel-based scaffold and pulsed electromagnetic field. Bioact Mater 22:312-324 (2023). PubMed: 36263100
- Zhang Y et al. Runx1 is a key regulator of articular cartilage homeostasis by orchestrating YAP, TGFβ, and Wnt signaling in articular cartilage formation and osteoarthritis. Bone Res 10:63 (2022). PubMed: 36307389
- Cho KH et al. Lactobacillus (LA-1) and butyrate inhibit osteoarthritis by controlling autophagy and inflammatory cell death of chondrocytes. Front Immunol 13:930511 (2022). PubMed: 36325344
- Zhou Z et al. The mitochondrial unfolded protein response (UPRmt) protects against osteoarthritis. Exp Mol Med 54:1979-1990 (2022). PubMed: 36380018