Anti-MMP13抗体[181-15A12] (ab77949)

概述

  • 产品名称Anti-MMP13抗体[181-15A12]
    参阅全部 MMP13 一抗
  • 描述
    小鼠单克隆抗体[181-15A12] to MMP13
  • 特异性Reacts with precursor and active forms of human MMP13 and does not cross react with human MMP1, 2, 3, 7, 8, 9.
  • 经测试应用适用于: Sandwich ELISA, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human matrix metalloproteinase 13 (human MMP13)

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab77949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Sandwich ELISA Use a concentration of 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to MMP13 (ab39012). For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab39012 as Detection.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • 组织特异性Seems to be specific to breast carcinomas.
  • 疾病相关Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CLG 3 antibody
    • CLG3 antibody
    • Collagenase 3 antibody
    • Collagenase3 antibody
    • MANDP1 antibody
    • Matrix metallopeptidase 13 (collagenase 3) antibody
    • Matrix Metalloproteinase 13 antibody
    • Matrix metalloproteinase-13 antibody
    • MMP 13 antibody
    • MMP-13 antibody
    • Mmp13 antibody
    • MMP13_HUMAN antibody
    see all

Anti-MMP13 antibody [181-15A12] 图像

实验方案

Anti-MMP13 antibody [181-15A12] (ab77949)参考文献

ab77949 has not yet been referenced specifically in any publications.

Product Wall

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I have attached the Abcam protocols books that contains some good IHC-P protocols, which contains some good general starting protocols. Specifically for ab39370, we recommend a starting primary antibody concentr...

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