概述

  • 产品名称Anti-MMP12抗体
    参阅全部 MMP12 一抗
  • 描述
    山羊多克隆抗体to MMP12
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog
  • 免疫原

    Synthetic peptide:

    C-RPKTSVNLISS

    , corresponding to internal sequence amino acids 313-323 of Human MMP12 (NP_002417.2)

  • 阳性对照
    • Human breast cancer cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab99473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).

靶标

  • 功能May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • 组织特异性Found in alveolar macrophages but not in peripheral blood monocytes.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 形式The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
  • 别名
    • EC 3.4.24.65 antibody
    • HME antibody
    • Macrophage elastase antibody
    • Macrophage metalloelastase antibody
    • Macrophage metaloelastase antibody
    • Matrix metallopeptidase 12 (macrophage elastase) antibody
    • Matrix metalloprotease 12 antibody
    • Matrix metalloproteinase-12 antibody
    • ME antibody
    • MGC138506 antibody
    • MME antibody
    • MMP 12 antibody
    • MMP-12 antibody
    • Mmp12 antibody
    • MMP12_HUMAN antibody
    see all

Anti-MMP12 antibody 图像

  • Anti-MMP12 antibody (ab99473) at 0.5 µg/ml + Human breast cancer lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 54 kDa

Anti-MMP12 antibody (ab99473)参考文献

ab99473 has not yet been referenced specifically in any publications.

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