Anti-MMP12抗体(ab231109)
Key features and details
- Rabbit polyclonal to MMP12
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
概述
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产品名称
Anti-MMP12抗体
参阅全部 MMP12 一抗 -
描述
兔多克隆抗体to MMP12 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Recombinant fragment (His-tag) corresponding to Rat MMP12 aa 350-500 (N terminal). (expressed in E.coli)
Database link: Q63341 -
阳性对照
- WB: Rat cerebrum lysate; mouse cerebrum lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Followed by Protein A affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab231109于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 54 kDa.
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说明 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 54 kDa. |
靶标
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功能
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
组织特异性
Found in alveolar macrophages but not in peripheral blood monocytes. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 17381 Mouse
- Entrez Gene: 117033 Rat
- SwissProt: P34960 Mouse
- SwissProt: Q63341 Rat
- Unigene: 2055 Mouse
- Unigene: 33193 Rat
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形式
The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus. -
别名
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
图片
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Anti-MMP12 antibody (ab231109) at 2 µg/ml + Recombinant rat MMP12
Developed using the ECL technique.
Predicted band size: 54 kDa -
Anti-MMP12 antibody (ab231109) at 2 µg/ml + Mouse cerebrum lysate
Developed using the ECL technique.
Predicted band size: 54 kDa -
Anti-MMP12 antibody (ab231109) at 2 µg/ml + Rat cerebrum lysate
Developed using the ECL technique.
Predicted band size: 54 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab231109 尚未被引用在任何文献中。