Anti-MMP12抗体(ab137443)
Key features and details
- Rabbit polyclonal to MMP12
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MMP12抗体
参阅全部 MMP12 一抗 -
描述
兔多克隆抗体to MMP12 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to a region within internal sequence amino acids 213 - 307 of Human MMP12 (NP_002417).
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阳性对照
- 293T, H1299 and HelaS3 whole cell lysates; DLD1 xenograft tissue; H1299 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137443于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
组织特异性
Found in alveolar macrophages but not in peripheral blood monocytes. -
序列相似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
结构域
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 4321 Human
- Omim: 601046 Human
- SwissProt: P39900 Human
- Unigene: 1695 Human
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形式
The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus. -
别名
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
图片
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Anti-MMP12 antibody (ab137443) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS PAGE -
Immunohistochemical analysis of parafin-embedded DLD1 xenograft tissue labelling MMP12 with ab137443 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed H1299 cells labelling MMP12 with ab137443 at 1/500 dilution. The image in the lower panel is costained with Hoechst 33342.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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