The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: Use at a concentration of 1 - 2 µg/ml. Antigen retrieval is not required.
IP: Use at 2µg/mg of lysate. Native verified. Use protein G.
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects bands of approximately ~60kDa (latent), ~47kDa (active) and ~35kDa (degradation product).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能May play an important role in the progression of epithelial malignancies.
组织特异性Specifically expressed in stromal cells of breast carcinomas.
序列相似性Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻译后修饰The precursor is cleaved by a furin endopeptidase.
细胞定位Secreted > extracellular space > extracellular matrix.
Immunohistochemistry analysis of formalin/fixed, paraffin-embedded Human placenta tissue, using the unconjugated version of ab79470, at 2.0µg/ml, with a peroxidase-conjugated secondary antibody and an AEC chromogen.
Note cytoplasmic staining of trophoblasts.