RabMAb

Anti-MMP11抗体[EP1259Y] (ab52904)

概述

  • 产品名称Anti-MMP11抗体[EP1259Y]
    参阅全部 MMP11 一抗
  • 描述
    兔单克隆抗体[EP1259Y] to MMP11
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human MMP11.

  • 阳性对照
    • MCF-7 cell lysate, human breast carcinoma tissue or MCF7 cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab52904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
WB 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
IHC-P Use at an assay dependent concentration.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能May play an important role in the progression of epithelial malignancies.
    • 组织特异性Specifically expressed in stromal cells of breast carcinomas.
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰The precursor is cleaved by a furin endopeptidase.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Matrix Metalloproteinase 11 antibody
      • Matrix metalloproteinase-11 antibody
      • MMP-11 antibody
      • Mmp11 antibody
      • MMP11_HUMAN antibody
      • SL 3 antibody
      • SL-3 antibody
      • SL3 antibody
      • ST3 antibody
      • STMY3 antibody
      • Stromelysin 3 antibody
      • Stromelysin III antibody
      • Stromelysin-3 antibody
      see all

    Anti-MMP11 antibody [EP1259Y] 图像

    • Anti-MMP11 antibody [EP1259Y] (ab52904) at 1/500 dilution + MCF-7 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled. at 1/2000 dilution

      Predicted band size : 55 kDa
      Observed band size : 55 kDa
    • Ab52904 (1:100) staining human MMP11 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunofluorescent staining of MCF7 cells using ab52904 (1:100).

    Anti-MMP11 antibody [EP1259Y] (ab52904)参考文献

    This product has been referenced in:
    • Yonemori M  et al. Dual tumor-suppressors miR-139-5p and miR-139-3p targeting matrix metalloprotease 11 in bladder cancer. Cancer Sci 107:1233-42 (2016). WB ; Human . Read more (PubMed: 27355528) »
    • Roscilli G  et al. Circulating MMP11 and specific antibody immune response in breast and prostate cancer patients. J Transl Med 12:54 (2014). IHC-P ; Human . Read more (PubMed: 24564996) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Human Tissue sections (Breast carcinoma)
    Specification Breast carcinoma
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    提交于 Jun 12 2013

    Thank you for your telephone call earlier this afternoon. I am very pleased to hear you would like to accept our offer and test ab52904 in IHC-Fr.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"