The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml.
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa).
功能May play an important role in the progression of epithelial malignancies.
组织特异性Specifically expressed in stromal cells of breast carcinomas.
序列相似性Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
翻译后修饰The precursor is cleaved by a furin endopeptidase.
细胞定位Secreted > extracellular space > extracellular matrix.
Ab53143 staining Human normal placenta. Staining is localised to the extracellular matrix (secreted). Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Anti-MMP11 antibody (ab53143)参考文献
This product has been referenced in:
Roscilli G et al. Circulating MMP11 and specific antibody immune response in breast and prostate cancer patients. J Transl Med12:54 (2014).
Read more (PubMed: 24564996) »