MMP10 overexpression 293T裂解液(全细胞) (ab94294)

概述

  • 产品名称MMP10 overexpression 293T裂解液(全细胞)
  • 描述
    MMP10 overexpression裂解液
  • 常规说明ab94294 is a 293T cell transfected lysate in which Human MMP10 has been transiently over-expressed using a pCMV-MMP10 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Domain: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. Function: Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

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应用

Our Abpromise guarantee covers the use of ab94294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

MMP10 overexpression 293T lysate (whole cell) 图像

  • ab94294 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-MMP10 antibody (ab89638) at 1/500 dilution

    Lane 1 : MMP10 overexpression 293T lysate (whole cell) (ab94294)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

MMP10 overexpression 293T lysate (whole cell) (ab94294)参考文献

ab94294 has not yet been referenced specifically in any publications.

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