概述

  • 产品名称
  • 描述
    兔多克隆抗体to MMP10
  • 特异性
    Detects endogenous levels of total MMP10 protein.
  • 经测试应用
    适用于: ICC/IF, ELISA, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) from an internal region of MMP10.

  • 阳性对照
    • HepG2 cells.

性能

应用

Our Abpromise guarantee covers the use of ab59437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration. PubMed: 21133852ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration. PubMed: 21133852

靶标

  • 功能
    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
  • 序列相似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Matrix metallopeptidase 10 (stromelysin 2) antibody
    • Matrix metalloprotease 10 antibody
    • Matrix metalloproteinase 10 (stromelysin 2) antibody
    • Matrix metalloproteinase 10 antibody
    • Matrix metalloproteinase-10 antibody
    • MMP 10 antibody
    • MMP-10 antibody
    • Mmp10 antibody
    • MMP10_HUMAN antibody
    • SL 2 antibody
    • SL-2 antibody
    • SL2 antibody
    • STMY 2 antibody
    • STMY2 antibody
    • Stromelysin 2 antibody
    • Stromelysin II antibody
    • Stromelysin-2 antibody
    • Stromelysin2 antibody
    • StromelysinII antibody
    • Transin 2 antibody
    • Transin-2 antibody
    • Transin2 antibody
    see all

图片

  • Immunofluorescent analysis of MMP10 in HepG2 cells with ab59437 (1/500 dilution); in the presence or absence of immunizing peptide.

文献

This product has been referenced in:
  • Clark RT  et al. T cell production of matrix metalloproteases and inhibition of parasite clearance by TIMP-1 during chronic Toxoplasma infection in the brain. ASN Neuro : (2010). Flow Cyt, IHC-Fr ; Mouse . Read more (PubMed: 21133852) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (First trimester decidua)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - 0.5% Triton
Specification
First trimester decidua
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Ruhul Choudhury

Verified customer

提交于 Jan 25 2017

Thank you very much for your interest in Abcam and our products..

To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册