概述

  • 产品名称Anti-MMP10抗体
    参阅全部 MMP10 一抗
  • 描述
    兔多克隆抗体to MMP10
  • 特异性The antibody binds to the hinge region of MMP10. The antibody binds to Stromelysin 2, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc.). The antibody also recognizes the cleaved active enzyme and will also bind to the non-reduced protein.
  • 经测试应用适用于: IHC-P, WB, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide based on the hinge region of the human MMP10 sequence.

    (Peptide available as ab41075.)

性能

应用

Our Abpromise guarantee covers the use of ab38930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
WB
IHC-Fr
  • 应用说明IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP10 is well conserved, higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein the antibody identifies bands at 56 kDa (the pro-form), as well as the initial active forms (46 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Matrix metallopeptidase 10 (stromelysin 2) antibody
      • Matrix metalloprotease 10 antibody
      • Matrix metalloproteinase 10 (stromelysin 2) antibody
      • Matrix metalloproteinase 10 antibody
      • Matrix metalloproteinase-10 antibody
      • MMP 10 antibody
      • MMP-10 antibody
      • Mmp10 antibody
      • MMP10_HUMAN antibody
      • SL 2 antibody
      • SL-2 antibody
      • SL2 antibody
      • STMY 2 antibody
      • STMY2 antibody
      • Stromelysin 2 antibody
      • Stromelysin II antibody
      • Stromelysin-2 antibody
      • Stromelysin2 antibody
      • StromelysinII antibody
      • Transin 2 antibody
      • Transin-2 antibody
      • Transin2 antibody
      see all

    Anti-MMP10 antibody 图像

    • Ab38930 staining Human normal trachea. Staining is localised to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    Anti-MMP10 antibody (ab38930)参考文献

    This product has been referenced in:
    • Zhang G  et al. Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature. Diagn Pathol 9:200 (2014). IHC ; Human . Read more (PubMed: 25387487) »
    • Frederick LA  et al. Matrix metalloproteinase-10 is a critical effector of protein kinase Ciota-Par6alpha-mediated lung cancer. Oncogene 27:4841-53 (2008). IHC-P . Read more (PubMed: 18427549) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    Application Western blot
    Sample Human Cell lysate - whole cell (endothelial cells)
    Loading amount 5000 cells
    Specification endothelial cells
    Gel Running Conditions Reduced Denaturing (10% acrylamide gel)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 01 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue lysate - whole (Human Skin)
    Specification Human Skin
    Fixative Methanol
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 0.02
    Username

    Abcam user community

    Verified customer

    提交于 Aug 26 2005

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"