RabMAb

Anti-MMP1抗体[EP1247Y] (ab52631)

概述

  • 产品名称Anti-MMP1抗体[EP1247Y]
    参阅全部 MMP1 一抗
  • 描述
    兔单克隆抗体[EP1247Y] to MMP1
  • 特异性The antibody is able to detect recombinant protein in western blot but it failed to detect the endogenous protein. Therefore, we do not recommend the antibody in this application. For western blot application we recommend using ab134184.
  • 经测试应用适用于: ICC/IF, IHC-Fr, Flow Cyt, IHC-Pmore details
    不适用于: IP or WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP1 aa 100-200.

  • 阳性对照
    • IHC-P: Human testis and cervical carcinoma tissues. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HeLa cells.
  • 常规说明

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab52631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
IHC-Fr Use at an assay dependent concentration. PubMed: 20220088
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • 应用说明Is unsuitable for IP or WB.
  • 靶标

    • 功能Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
    • 序列相似性Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • 结构域There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • 翻译后修饰Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
    • 细胞定位Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 27 kDa interstitial collagenase antibody
      • CLG antibody
      • CLGN antibody
      • collagenase, fibroblast antibody
      • collagenase, interstitial antibody
      • Fibroblast collagenase antibody
      • Interstitial collagenase antibody
      • Matrix metallopeptidase 1 (interstitial collagenase) antibody
      • Matrix metalloprotease 1 antibody
      • Matrix Metalloproteinase 1 antibody
      • Matrix metalloproteinase-1 antibody
      • MMP 1 antibody
      • MMP-1 antibody
      • MMP1 antibody
      • MMP1_HUMAN antibody
      • OTTHUMP00000045866 antibody
      see all

    Anti-MMP1 antibody [EP1247Y] 图像

    • Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of cervix tissue labeling MMP1 with ab52631 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counterstained with hematoxylin.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/30. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/30) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with unpurified ab52631 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

      Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with purified ab52631 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

    • Flow cytometry analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling MMP1 with ab52631 at 1/50.

    • ICC/IF image of unpurified ab52631 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52631, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Flow cytometry analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). Green - Isotype control, rabbit monoclonal IgG.

    Anti-MMP1 antibody [EP1247Y] (ab52631)参考文献

    This product has been referenced in:
    • Kotula E  et al. DNA-PKcs plays role in cancer metastasis through regulation of secreted proteins involved in migration and invasion. Cell Cycle 14:1961-72 (2015). WB . Read more (PubMed: 26017556) »
    • Tewari A  et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF ; Human . Read more (PubMed: 24714202) »

    See all 12 Publications for this product

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    Thank you for contacting us.

    The immunogen sequence homology of antibody ab52631 with bovine is about 80%. There are chances that this antibody will cross react however because we do not have tested data so we cannot confirm it. We are much mo...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Tissue lysate - whole (human colon (mucosa))
    Loading amount 40 µg
    Specification human colon (mucosa)
    Gel Running Conditions Reduced Denaturing (10)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
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    Verified customer

    提交于 May 15 2012

    Thank you for contacting us. We would suggest using PBS with 1% BSA as a diluting buffer. Please be advised that if you would like to store these antibodies at -20 or as recommended on the datasheet then we would recommend storing the antibodie...

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    After investigation with the laboratory, I can confirm we have not extensively tested this antibody against all other MMP family members, this antibody, along with the other MMP antibodies should be specific for its particular target. Also, ab52631 sho...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"