概述

  • 产品名称
  • 描述
    兔多克隆抗体to MMP1
  • 特异性
    This antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9).
  • 经测试应用
    适用于: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 种属反应性
    与反应: Horse, Dog, Human
  • 免疫原

    Synthetic peptide corresponding to MMP1. based on the hemopexin domain of the human sequence
    (Peptide available as ab41003)

性能

应用

Our Abpromise guarantee covers the use of ab38929 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 18434409
IHC-Fr Use at an assay dependent concentration.
WB 1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.

靶标

  • 功能
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 序列相似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域
    There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰
    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • 细胞定位
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

图片

  • All lanes : Anti-MMP1 antibody (ab38929)

    Lane 1 : Human MMP1.
    Lane 2 : Cell Media from human chondrosarcoma (no treatment).
    Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).


    Predicted band size : 54 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)
  • ab47116 (2µg/ml) staining Zinc Alpha 2 Glycoprotein in human breast tumour using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in Myoepithelial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ab38929 staining MMP1 in Horse fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 0.1% serum for 30 minutes. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted FITC-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin stained red and nuclear stained in blue with DAPI

    See Abreview

文献

This product has been referenced in:
  • McKay TB  et al. Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro. PLoS One 12:e0176017 (2017). WB ; Human . Read more (PubMed: 28426715) »
  • Shen CJ  et al. MMP1 expression is activated by Slug and enhances multi-drug resistance (MDR) in breast cancer. PLoS One 12:e0174487 (2017). WB ; Human . Read more (PubMed: 28334049) »

See all 12 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A549)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
60 µg
Specification
A549
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 Apr 06 2016

The laboratory that produces this antibody reports that it is unlikely to cross-react with mouse MMP1:

"Mice make 2 forms of MMP-1, the A form (NP_114395.1) and the B form (NP_114396.3), although the proteins have long been thought to be mad...

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Thank you for contacting us.

Please find the information below describingour testing discount program:

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Loading amount
100000 cells
Specification
MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 23 2012

Thank you for your enquiry. I can confirm that ab38929 MMP1 antibody is known to detect both pro and active forms of MMP1. I hope this is helpful to you. Should you have any further questions, please do not hesitate to contact us.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Horse Tissue lysate - other (Cartilage explant culture secretome)
Loading amount
50 µg
Specification
Cartilage explant culture secretome
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Ali Mobasheri

Verified customer

提交于 Jan 20 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (human fetal heart)
Specification
human fetal heart
Fixative
Paraformaldehyde
Permeabilization
Yes - saponin 0.1% in PBS
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 37°C
Username

Abcam user community

Verified customer

提交于 May 29 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Horse Cell (fibroblast)
Specification
fibroblast
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.1%
Username

Dr. Yuan ZhengQiang

Verified customer

提交于 Apr 03 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (skin tumor)
Specification
skin tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 0.1%
Username

Abcam user community

Verified customer

提交于 Mar 30 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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