概述

  • 产品名称Anti-MMP1抗体
    参阅全部 MMP1 一抗
  • 描述
    兔多克隆抗体to MMP1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide KLH conjugated, from the N-terminus of the pro form of Human MMP1 (NP_002412.1).

  • 阳性对照
    • Human Kidney and Prostate tissues.

性能

应用

Our Abpromise guarantee covers the use of ab118529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

Anti-MMP1 antibody 图像

  • Human Kidney tissue (formalin-fixed, paraffin-embedded) stained with ab118529 at 5 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Human Prostate tissue (formalin-fixed, paraffin-embedded) stained with ab118529 at 5 µg/ml followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-MMP1 antibody (ab118529)参考文献

This product has been referenced in:
  • Sun Y  et al. Effect of pERK2 on extracellular matrix turnover of the fibrotic joint capsule in a post-traumatic joint contracture model. Exp Ther Med 11:547-552 (2016). IHC-Fr ; Rat . Read more (PubMed: 26893645) »

See 1 Publication for this product

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To our knowledge, ab118529 has not been experimentally tested in rat. However by participating in our AbTrial program you can now use our products in an untested application or speci...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"