MMP-3 Activity Assay试剂盒(Fluorometric) (ab118972)

概述

  • 产品名称MMP-3 Activity Assay试剂盒(Fluorometric)
    参阅全部 MMP3 试剂盒
  • 检测方法Fluorescent
  • 测试
    100 x 1 assay
  • 样品类型
    Cell culture supernatant, Milk, Urine, Tissue Extracts, Cell culture media
  • 检测类型Enzyme activity
  • 灵敏度
    < 0.05 mU/well
  • 检测时间
    1h 00m
  • 种属反应性
    与反应: all Mammals
  • 产品概述

    In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.

  • 经测试应用适用于: Functional Studiesmore details

性能

  • 功能Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • CHDS6
    • Matrix metalloproteinase 3
    • Matrix metalloproteinase-3
    • MGC126102
    • MGC126103
    • MGC126104
    • MMP 3
    • MMP-3
    • MMP3
    • MMP3_HUMAN
    • Proteoglycanase
    • SL-1
    • SL1
    • STMY
    • STMY1
    • STR1
    • Stromelysin 1
    • Stromelysin-1
    • Transin 1
    • Transin-1
    see all

应用

Our Abpromise guarantee covers the use of ab118972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

MMP-3 Activity Assay Kit (Fluorometric) 图像

  • MMP-3 Mca Standard
  • MMP-3 Positive Control

实验方案

MMP-3 Activity Assay Kit (Fluorometric) (ab118972)参考文献

This product has been referenced in:
  • Pettersson F  et al. Genetic and pharmacologic inhibition of eIF4E reduces breast cancer cell migration, invasion, and metastasis. Cancer Res 75:1102-12 (2015). Read more (PubMed: 25608710) »

See 1 Publication for this product

Product Wall

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Since ab118972 is an enzyme assay, we would not recommend EDTA treated plasma for it. The customer could try using heparin instead for plasma.

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The FRET substrate we provide in this kit is very specific for MMP-3. Other MMPs should not be able to hydrolyze it, therefore there should not be any interference of that sort.

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EDTA is known to inhibit MMP-3 activity in general conditions. However, we have not encountered this problem specifically. It might depend on the concentration of EDTA used. Nevertheless, it is always better to u...

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The molecular weight of the Active MMP-3 is: 19.5 kDa.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us. Each vial of MMP-3 positive control contains 2.2 ug of the active protein that has been lyophilized. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or infor...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"