MMP-1 Inhibitor Screening试剂盒(ab118973)


  • 产品名称MMP-1 Inhibitor Screening试剂盒
    参阅全部 MMP1 试剂盒
  • 测试
    100 x 1 assay
  • 检测类型Quantitative
  • 检测时间
    0h 45m
  • 产品概述

    In Abcam's MMP-1 Inhibitor Screening Kit, MMP-1 hydrolyzes a specific FRET substrate to release a quenched fluorescent group, which can be detected at Em/Ex = 490/520nm. In presence of potent MMP-1 inhibitors the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of MMP-1 inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, GM 6001 (IC50 = 0.4 nM for MMP-1) is included.
    Visit our FAQs page for tips and troubleshooting.

  • 说明

    The Matrix metalloproteinase-1 (MMP-1, Interstitial collagenase, fibroblast collagenase) is a member of a multigene family of calcium-dependent, zinc-containing endoproteinases, the matrix metalloproteinases (MMPs). The MMPs are responsible for the degradation of the extracellular matrix (ECM) including collagens, elastins, gelatin, matrix glycoproteins and proteoglycan during normal development and disease processes. MMPs are regulated by hormones, growth factors and cytokines. MMP-1 belongs to the subclass, the collagenases, and along with MMP-8, and MMP-13 are the only members of the MMP family that are capable of degrading the types I, II and III interstitial collagens with high efficiency. These collagens are primarily found in bone, cartilage and skin.

  • 经测试应用适用于: Functional Studiesmore details


  • 功能Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 序列相似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • 结构域There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻译后修饰Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • 细胞定位Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 别名
    • 27 kDa interstitial collagenase
    • CLG
    • CLGN
    • Fibroblast collagenase
    • Interstitial collagenase
    • Matrix metallopeptidase 1
    • Matrix metallopeptidase 1 (interstitial collagenase)
    • Matrix metalloprotease 1
    • Matrix metalloproteinase 1
    • Matrix metalloproteinase-1
    • MMP 1
    • MMP-1
    • MMP1
    • MMP1_HUMAN
    see all


Our Abpromise guarantee covers the use of ab118973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

MMP-1 Inhibitor Screening Kit 图像

  • MMP-1 Activity Profile at Various Concentrations of GM 6001
  • MMP-1 Inhibition by GM 6001


MMP-1 Inhibitor Screening Kit (ab118973)参考文献

ab118973 has not yet been referenced specifically in any publications.

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