Anti-MMD2抗体(ab72706)

概述

  • 产品名称Anti-MMD2抗体
    参阅全部 MMD2 一抗
  • 描述
    小鼠多克隆抗体to MMD2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length human MMD2 (NP_940685.2, 246 a.a)

  • 阳性对照
    • Human kidney tissue lysate, MMD2 transfected 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 31 , 29 kDa). (isoform 1 and isoform 2, respectively.)

靶标

  • 相关性MMD2 is a homologue of MMD and both proteins are members of the so far uncharacterized Hemolysin-III (Hly-III) family, and are atypical members of the PAQR family. PAQR10/MMD2, has recently (Góñez et al.Nov/Dec 2008) been found to be expressed in mouse pancreatic islet beta cells, and may have a role in the endocrine pancreas development and hyperplasia in pregnancy.
  • 细胞定位Golgi apparatus membrane; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • FLJ37205 antibody
    • MMD 2 antibody
    • Monocyte to macrophage differentiation associated 2 antibody
    • Monocyte to macrophage differentiation associated protein 2 antibody
    • Monocyte to macrophage differentiation factor 2 antibody
    • PAQR10 antibody
    • Progestin and adipoQ receptor family member 10 antibody
    • Progestin and adipoQ receptor family member X antibody
    see all

Anti-MMD2 antibody 图像

  • Anti-MMD2 antibody (ab72706) at 1/500 dilution + human kidney lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 , 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-MMD2 antibody (ab72706) at 1/500 dilution

    Lane 1 : MMD2 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 31 , 29 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

Anti-MMD2 antibody (ab72706)参考文献

ab72706 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (15P1)
Specification 15P1
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% saponin
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
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Verified customer

提交于 Feb 28 2012

You are very welcome! Have a great day.

Yes, you can place the order (both this one and the one for the free primary) as usual through the distributor. The expiration date is four months after issuing the code (see below), however, in individual cases we may be able to make an excemption and...

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Thank you very much for your interest in ab72706. So far we have tested ab72706 only in Western blot with human samples. However, the sequence similarity between the human and the murine MMD2 is 87% and a cross-reactivity of this antibody is likely...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"