Anti-MMACHC/CblC抗体(ab96195)
Key features and details
- Rabbit polyclonal to MMACHC/CblC
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-MMACHC/CblC抗体
参阅全部 MMACHC/CblC 一抗 -
描述
兔多克隆抗体to MMACHC/CblC -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow -
免疫原
Recombinant fragment corresponding to Human MMACHC/CblC aa 1-188.
Database link: NP_056321 -
阳性对照
- 293T and Raji whole cell lysates; HeLa cells; A431, H1299, HeLaS3 and HepG2 cell lines
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96195于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
-
功能
May be involved in the binding and intracellular trafficking of cobalamin (vitamin B12). -
组织特异性
Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes. -
通路
Cofactor biosynthesis; adenosylcobalamin biosynthesis. -
疾病相关
Defects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood. -
序列相似性
Belongs to the MMACHC family. - Information by UniProt
-
数据库链接
- Entrez Gene: 513433 Cow
- Entrez Gene: 25974 Human
- Entrez Gene: 67096 Mouse
- Entrez Gene: 313520 Rat
- Omim: 609831 Human
- SwissProt: Q5E9C8 Cow
- SwissProt: Q9Y4U1 Human
- SwissProt: Q9CZD0 Mouse
see all -
别名
- 1810037K07Rik antibody
- BOS_3654 antibody
- cblC antibody
see all
图片
-
All lanes : Anti-MMACHC/CblC antibody (ab96195) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS-PAGE -
ab96195, at 1/100 dilution, staining MMACHC/CblC in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab96195 被引用在 2 文献中.
- Fofou-Caillierez MB et al. Interaction between methionine synthase isoforms and MMACHC: characterization in cblG-variant, cblG and cblC inherited causes of megaloblastic anaemia. Hum Mol Genet N/A:N/A (2013). PubMed: 23825108
- Yu HC et al. An X-linked cobalamin disorder caused by mutations in transcriptional coregulator HCFC1. Am J Hum Genet 93:506-14 (2013). PubMed: 24011988