概述

  • 产品名称Anti-MLX抗体
    参阅全部 MLX 一抗
  • 描述
    兔多克隆抗体to MLX
  • 特异性ab65147 detects endogenous levels of total MLX protein.
  • 经测试应用适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human MLX

  • 阳性对照
    • LOVO cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab65147 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
ELISA
WB
  • 应用说明ELISA: 1/10000.
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
    • 组织特异性Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.
    • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
    • 细胞定位Cytoplasm. Found predominantly in the cytoplasm and Nucleus. Found predominantly in the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • bHLHd13 antibody
      • BigMax protein antibody
      • Class D basic helix-loop-helix protein 13 antibody
      • MAD7 antibody
      • Max like bHLHZip protein antibody
      • Max like protein X antibody
      • Max-like bHLHZip protein antibody
      • Max-like protein X antibody
      • MLX antibody
      • MLX_HUMAN antibody
      • MLXIP antibody
      • MONDOA antibody
      • MXD7 antibody
      • Protein BigMax antibody
      • Protein Mlx antibody
      • TCFL4 antibody
      • transcription factor like 4 antibody
      • Transcription factor like protein 4 antibody
      • Transcription factor-like protein 4 antibody
      see all

    Anti-MLX antibody 图像

    • All lanes : Anti-MLX antibody (ab65147) at 1/500 dilution

      Lane 1 : LOVO cell extract
      Lane 2 : LOVO cell extract with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.


      Predicted band size : 33 kDa
      Observed band size : 33 kDa
    • ICC/IF image of ab65147 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65147, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-MLX antibody (ab65147)参考文献

    ab65147 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"