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Other Immunogen Type corresponding to MLN64. Recombinant MLN64 protein.
Our Abpromise guarantee covers the use of ab3478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|Inhibition Assay||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.|
ab3478 staining MLN64 in Human ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours 10 minutes at 4°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 20 minutes at 25°C. An undiluted HRP-conjugated monoclonal was used as the secondary antibody.
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