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Our Abpromise guarantee covers the use of ab75339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 205 kDa.|
|ICC||Use at an assay dependent dilution.|
ab75339 staining MLL5 in HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in Methanol and permeabilized in PHEM/0.5% Triton X-100 prior to blocking in 2.5% BSA for 1 hour at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. The secondary antibody was FITC-conjugated Donkey anti-Rabbit polyclonal, diluted 1/150.
DNA (DAPI) stain in blue, anti-Aurora A antibody in red, anti-MLL5 in green. Left cell shows centrosomal staining in interphase (arrow head). Right cell shows centrosomal staining in mitotic cell. Centrosomal localisation of MLL5 was shown in Liu et al (2010) PMID:20439461
Image courtesy of an anonymous Abreview.Fujiki et al (2010) (PMID:19377461) report two isoforms of MLL5 at 75 and 260 kDa corresponding to the major bands seen with this antibody.
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