Anti-MLL5抗体(ab192582)
Key features and details
- Rabbit polyclonal to MLL5
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-MLL5抗体 -
描述
兔多克隆抗体to MLL5 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein corresponding to Human MLL5 aa 1 to the C-terminus.
Database link: Q8IZD2 -
阳性对照
- WB: NCI-H460 and HEK-293T cell lysates and Mouse brain tissue lysate.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab192582 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab192582于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Predicted molecular weight: 205 kDa.
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说明 |
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WB
1/500. Predicted molecular weight: 205 kDa. |
靶标
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功能
Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic-acid-induced granulopoiesis by acting as a coactivator of RAR-alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells. Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases. -
组织特异性
Widely expressed in both adult and fetal tissues. Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum. -
序列相似性
Belongs to the histone-lysine methyltransferase family. TRX/MLL subfamily.
Contains 1 PHD-type zinc finger.
Contains 1 SET domain. -
翻译后修饰
O-glycosylation at Thr-440 in the SET domain by OGT is essential for the histone methyltransferase and the coactivator activity toward RARA in granulopoiesis. The absence of Thr-440 glycosylation in assays done in vitro may explain why some authors did not detected any histone methyltransferase activity for this protein. -
细胞定位
Nucleus speckle. Absent from the nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 55904 Human
- Entrez Gene: 69188 Mouse
- Omim: 608444 Human
- SwissProt: Q8IZD2 Human
- SwissProt: Q3UG20 Mouse
- Unigene: 592262 Human
- Unigene: 205190 Mouse
- Unigene: 403814 Mouse
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别名
- HDCMC04P antibody
- Histone lysine N methyltransferase MLL5 antibody
- Histone-lysine N-methyltransferase MLL5 antibody
see all
图片
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All lanes : Anti-MLL5 antibody (ab192582) at 1/500 dilution
Lane 1 : NCI-H460 (human lung cancer cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Mouse brain tissue lysate
Predicted band size: 205 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab192582 尚未被引用在任何文献中。