Anti-MLK4抗体- N-terminal (ab71510)

概述

  • 产品名称Anti-MLK4抗体- N-terminal
    参阅全部 MLK4 一抗
  • 描述
    兔多克隆抗体to MLK4 - N-terminal
  • 经测试应用适用于: ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide from the N-terminal region of human MLK4 conjugated to KLH.

  • 阳性对照
    • 293 cell lysates transiently transfected with the MLK4 gene. Human colon carcinoma tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明ab71510 is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab71510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/10 - 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 114 kDa.

靶标

  • 功能Activates the JUN N-terminal pathway.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • 翻译后修饰Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • dJ862P8.3 antibody
    • KIAA1804 antibody
    • M3KL4_HUMAN antibody
    • Mitogen activated protein kinase kinase kinase antibody
    • Mitogen-activated protein kinase kinase kinase MLK4 antibody
    • Mixed lineage kinase 4 antibody
    • MLK 4 antibody
    • Mlk4 antibody
    • RP5 862P8.2 antibody
    see all

Anti-MLK4 antibody - N-terminal 图像

  • All lanes : Anti-MLK4 antibody - N-terminal (ab71510) at 1/50 dilution

    Lane 1 : Nontransfected 293 cell lysate
    Lane 2 : 293 cell lysate transiently transfected with the MLK4 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 114 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa,50 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • ab71510 at 1/10 dilution, staining MLK4 in human colon carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-MLK4 antibody - N-terminal (ab71510)参考文献

ab71510 has not yet been referenced specifically in any publications.

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