概述

  • 产品名称Anti-MLK3抗体[EP1460Y]
    参阅全部 MLK3 一抗
  • 描述
    兔单克隆抗体[EP1460Y] to MLK3
  • 经测试应用适用于: Flow Cyt, WB, ICC/IFmore details
    不适用于: IHC-Fr,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus of human MLK3.

  • 阳性对照
    • A431 cell lysate or HeLa cells.
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab51068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/15.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for IHC-Fr,IHC-P or IP.
  • 靶标

    • 功能Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
    • 组织特异性Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
    • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH3 domain.
    • 翻译后修饰Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 2610017K16Rik antibody
      • EC 2.7.11.25 antibody
      • M3K11_HUMAN antibody
      • Map3k11 antibody
      • MEKK11 antibody
      • MGC17114 antibody
      • Mitogen-activated protein kinase kinase kinase 11 antibody
      • Mixed lineage kinase 3 antibody
      • Mixed lineage protein kinase 3 antibody
      • Mlk-3 antibody
      • Mlk3 antibody
      • Protein tyrosine kinase PTK1 antibody
      • PTK1 antibody
      • RHOE antibody
      • SH3 domain containing proline rich kinase antibody
      • SPRK antibody
      • Src-homology 3 domain-containing proline-rich kinase antibody
      see all

    Anti-MLK3 antibody [EP1460Y] 图像



    • Predicted band size : 93 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MLK3 knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51068 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab51068 was shown to specifically react with MLK3 when MLK3 knockout samples were used. Wild-type and MLK3 knockout samples were subjected to SDS-PAGE. ab51068 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-MLK3 antibody [EP1460Y] (ab51068) at 1/5000 dilution + A431 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 93 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells using ab51068 (1:100).

    Anti-MLK3 antibody [EP1460Y] (ab51068)参考文献

    This product has been referenced in:
    • Marusiak AA  et al. Mixed lineage kinases activate MEK independently of RAF to mediate resistance to RAF inhibitors. Nat Commun 5:3901 (2014). WB ; Human . Read more (PubMed: 24849047) »
    • Li CH  et al. Zinc oxide nanoparticles-induced intercellular adhesion molecule 1 expression requires Rac1/Cdc42, mixed lineage kinase 3, and c-Jun N-terminal kinase activation in endothelial cells. Toxicol Sci 126:162-72 (2012). Read more (PubMed: 22166487) »

    See all 5 Publications for this product

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