Anti-MLF1 Interacting蛋白/PBIP1 (phospho T78)抗体(ab115570)
概述
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产品名称Anti-MLF1 Interacting蛋白/PBIP1 (phospho T78)抗体
参阅全部 MLF1 Interacting Protein/PBIP1 一抗 -
描述兔多克隆抗体to MLF1 Interacting蛋白/PBIP1 (phospho T78)
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宿主Rabbit
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经测试应用适用于: WB, IP, ELISA, IHC-Pmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Chimpanzee
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免疫原
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acids surrounding Thr78 of Human MLF1 Interacting Protein/PBIP1.
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阳性对照
- Human prostate tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.27% Potassium phosphate -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab115570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/500 - 1/2000. Predicted molecular weight: 48 kDa. | |
| IP | Use at an assay dependent concentration. | |
| ELISA | 1/5000 - 1/25000. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
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组织特异性Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
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翻译后修饰Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
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细胞定位Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.
- Information by UniProt
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数据库链接
- Entrez Gene: 617866 Cow
- Entrez Gene: 79682 Human
- Entrez Gene: 71876 Mouse
- Entrez Gene: 306464 Rat
- Omim: 611511 Human
- SwissProt: Q2KIW5 Cow
- SwissProt: Q71F23 Human
- SwissProt: Q8C4M7 Mouse
see all -
别名
- CENP U antibody
- CENP-50 antibody
- CENP-U antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody (ab115570)Human Prostate (formalin-fixed, paraffin-embedded) stained with ab115570 at 10 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
文献
ab115570 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"