Anti-MLF1 Interacting蛋白/PBIP1 (phospho T78)抗体(ab115570)
Key features and details
- Rabbit polyclonal to MLF1 Interacting Protein/PBIP1 (phospho T78)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-MLF1 Interacting蛋白/PBIP1 (phospho T78)抗体 -
描述
兔多克隆抗体to MLF1 Interacting蛋白/PBIP1 (phospho T78) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Chimpanzee -
免疫原
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acids surrounding Thr78 of Human MLF1 Interacting Protein/PBIP1.
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阳性对照
- Human prostate tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab115570于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression. -
组织特异性
Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines. -
翻译后修饰
Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1. -
细胞定位
Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates. - Information by UniProt
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数据库链接
- Entrez Gene: 617866 Cow
- Entrez Gene: 79682 Human
- Entrez Gene: 71876 Mouse
- Entrez Gene: 306464 Rat
- Omim: 611511 Human
- SwissProt: Q2KIW5 Cow
- SwissProt: Q71F23 Human
- SwissProt: Q8C4M7 Mouse
see all -
别名
- CENP U antibody
- CENP-50 antibody
- CENP-U antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab115570 尚未被引用在任何文献中。