概述

  • 产品名称Anti-MLC1抗体
    参阅全部 MLC1 一抗
  • 描述
    兔多克隆抗体to MLC1
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Guinea pig, Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 180 - 229 (DSANILDEVP FPARVLKSYS VVEVIAGISA VLGGIIALNV DDSVSGPHLS) of Human MLC1 (NP_055981).

  • 阳性对照
    • Fetal spleen lysate

性能

应用

Our Abpromise guarantee covers the use of ab94730 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-MLC1 antibody 图像

  • ab94730 staining MLC1 in primary Rat cortical astrocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde. After 1 hour of incubation with blocking solution (5% BSA in PBS), cells were incubated for 1 hour at room temperature with a NBD phallacidin high-affinity F-actin probe (1/30) to stain actin filaments and visualize the whole cell body, used in combination with ab94730 (1/50) diluted in PBS+0.2% Triton X100.To amplify MLC1 staining, a biotinilated secondary antibody (4.3 µg/ml, Biotin-SP-AffiniPure goat anti-rabbit IgG H+L) followed by incubation with 2 µg/ml streptavidin-TRITC was used.

  • Anti-MLC1 antibody (ab94730) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal spleen lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa

    Gel concentration: 12%

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Anti-MLC1 antibody (ab94730) at 1/500 dilution + Mouse brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocked with 5% Milk for 1 hour at 25°C.

     

    Secondary antibody - anti-rabbit HRP (ab6721)

    See Abreview

Anti-MLC1 antibody (ab94730)参考文献

ab94730 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Tissue lysate - other (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jun 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"