概述

  • 产品名称Anti-MKRN2抗体
    参阅全部 MKRN2 一抗
  • 描述
    兔多克隆抗体to MKRN2
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Dog, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 366 and the C-terminus (residue 416) of human MKRN2 (NP_054879.3).

  • 阳性对照
    • Whole cell lysates from HeLa, 293T and NIH3T3 cells.

性能

应用

Our Abpromise guarantee covers the use of ab72055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 47 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

Anti-MKRN2 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MKRN2 with ab72055 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MKRN2 antibody (ab72055) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg


    Predicted band size : 47 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa. We are unsure as to the identity of these extra bands.
  • Detection of Human MKRN2 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72055 at 3 µg/mg lysate (Lane 1). Lane 2 represents Control IgG IP. Subsequent Western blot detection was done using ab72055 at 1 µg/ml.

Anti-MKRN2 antibody (ab72055)参考文献

ab72055 has not yet been referenced specifically in any publications.

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