Anti-MKRN1抗体(ab58768)

概述

  • 产品名称Anti-MKRN1抗体
    参阅全部 MKRN1 一抗
  • 描述
    兔多克隆抗体to MKRN1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: DNCRYSHDLS DSPYSVVCKY FQRGYCIYGD RCRYEHSKPL KQEEATATEL, corresponding to N terminal amino acids 73-122 of Human MKRN1

  • 阳性对照
    • Jurkat cell lysate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab58768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

靶标

  • 功能E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
  • 组织特异性Ubiquitous.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰Auto-ubiquitinated; which leads to proteasomal degradation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase makorin-1 antibody
    • FLJ21334 antibody
    • HGNC:7112 antibody
    • Makorin antibody
    • Makorin ring finger protein 1 antibody
    • MKRN 1 antibody
    • mkrn1 antibody
    • MKRN1_HUMAN antibody
    • OTTHUMP00000212117 antibody
    • OTTHUMP00000212118 antibody
    • OTTHUMP00000212121 antibody
    • OTTHUMP00000212124 antibody
    • Ring finger protein 1 antibody
    • RING finger protein 61 antibody
    • RNF61 antibody
    see all

Anti-MKRN1 antibody 图像

  • Anti-MKRN1 antibody (ab58768) at 1.25 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk in PBS buffer

    Secondary
    RP conjugated anti-Rabbit IgG at 50000

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

Anti-MKRN1 antibody (ab58768)参考文献

ab58768 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (DU145 Prostate Cancer Cells)
Loading amount 50 µg
Specification DU145 Prostate Cancer Cells
Treatment Control, POH, or rapamycin treated cells
Gel Running Conditions Reduced Denaturing (4-15% polyacrylamide TGX mini-gel (Bio-Rad))
Blocking step protein-free bløk-CH™ buffer (Millipore as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Dr. Tabetha Sundin

Verified customer

提交于 Aug 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"