概述

  • 产品名称Anti-MKRN1抗体
    参阅全部 MKRN1 一抗
  • 描述
    山羊多克隆抗体to MKRN1
  • 特异性ab123804 is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    RYEHSKPLKQEEAT

    by a Cysteine residue linker, corresponding to internal sequence amino acids 105-118 of Human MKRN1 (NP_038474.2; NP_001138597.1).

  • 阳性对照
    • MKRN1 transfected HEK293 cells.

性能

应用

Our Abpromise guarantee covers the use of ab123804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription.
  • 组织特异性Ubiquitous.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 4 C3H1-type zinc fingers.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰Auto-ubiquitinated; which leads to proteasomal degradation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • E3 ubiquitin-protein ligase makorin-1 antibody
    • FLJ21334 antibody
    • HGNC:7112 antibody
    • Makorin antibody
    • Makorin ring finger protein 1 antibody
    • MKRN 1 antibody
    • mkrn1 antibody
    • MKRN1_HUMAN antibody
    • OTTHUMP00000212117 antibody
    • OTTHUMP00000212118 antibody
    • OTTHUMP00000212121 antibody
    • OTTHUMP00000212124 antibody
    • Ring finger protein 1 antibody
    • RING finger protein 61 antibody
    • RNF61 antibody
    see all

Anti-MKRN1 antibody 图像

  • All lanes : Anti-MKRN1 antibody (ab123804) at 1 µg/ml

    Lane 1 : fetal mouse brain tissue lysate
    Lane 2 : adult mouse brain tissue lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 53 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa (possible isoform).
  • Lane 1 : Anti-MKRN1 antibody (ab123804) at 0.3 µg/ml
    Lane 2 : Anti-MKRN1 antibody (ab123804) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK Tag anntibody at 1/1000 dilution

    Lane 1 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
    Lane 2 : HEK293 lysate, mock-transfected
    Lane 3 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 53 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

Anti-MKRN1 antibody (ab123804)参考文献

ab123804 has not yet been referenced specifically in any publications.

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