This antibody gave a positive signal in HepG2 and HEK293 as well as the following tissue lysates: Mouse Lung; Human Placenta; Human Small Intestine.
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MEF1
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
Use a concentration of 5 µg/ml.
Transcriptional coactivator of serum response factor (SRF) with the potential to modulate SRF target genes. Suppresses TNF-induced cell death by inhibiting activation of caspases; its transcriptional activity is indispensable for the antiapoptotic function. It may up-regulate antiapoptotic molecules, which in turn inhibit caspase activation.
Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain.
Note=A chromosomal aberration involving MKL1 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with RBM15. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene.
Contains 2 RPEL repeats. Contains 1 SAP domain.
The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity. The RPEL repeats mediate binding to globular actin.
Cytoplasm. Nucleus. Binding to globular actin is required to maintain cytoplasmic localization.
ICC/IF image of ab115319 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab115319 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-Mkl1 antibody (ab115319)
All lanes : Anti-Mkl1 antibody (ab115319) at 1 µg/ml
Lane 1 : Mouse lung normal tissue lysate - total protein (ab29297) Lane 2 : Human placenta tissue lysate - total protein (ab29745) Lane 3 : Human small intestine tissue lysate - total protein (ab29276) Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 99 kDa Observed band size : 99 kDa Additional bands at : 22 kDa,35 kDa,65 kDa. We are unsure as to the identity of these extra bands.