Anti-Mixl1抗体(ab28411)

概述

  • 产品名称Anti-Mixl1抗体
    参阅全部 Mixl1 一抗
  • 描述
    兔多克隆抗体to Mixl1
  • 经测试应用适用于: WBmore details
  • 种属反应性

    预测可用于: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Mouse Mixl1.

    (Peptide available as ab30617.)

  • 阳性对照
    • This antibody gave a positive signal in Murine ES WCL expressing mesoderm Marker lysate.

性能

应用

Our Abpromise guarantee covers the use of ab28411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

靶标

  • 功能Transcription factor that play a central role in proper axial mesendoderm morphogenesis and endoderm formation. Required for efficient differentiation of cells from the primitive streak stage to blood, by acting early in the recruitment and/or expansion of mesodermal progenitors to the hemangioblastic and hematopoietic lineages. Also involved in the morphogenesis of the heart and the gut during embryogenesis. Acts as a negative regulator of brachyury expression.
  • 组织特异性Restricted to progenitors and secondary lymph tissues. In normal hematopoiesis, it is restricted to immature B-and T-lymphoid cells. Present in differentiating embryonic stem cells (at protein level).
  • 序列相似性Belongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰Phosphorylated at multiple sites.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hMix antibody
    • Homeobox protein MIXL1 antibody
    • Homeodomain protein 1 antibody
    • Homeodomain protein MIX antibody
    • MILD1 antibody
    • MIX antibody
    • Mix.1 antibody
    • Mix.1 homeobox-like protein antibody
    • Mix1 homeobox-like 1 antibody
    • MIX1 homeobox-like protein 1 antibody
    • MIXL antibody
    • Mixl1 antibody
    • MIXL1_HUMAN antibody
    see all

Anti-Mixl1 antibody 图像

  • Anti-Mixl1 antibody (ab28411) at 1 µg/ml + Whole Cell Lysate from Murine ES Cells Differentiated to Express Mesoderm Markers at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

Anti-Mixl1 antibody (ab28411)参考文献

ab28411 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Day 4 differentiated Mouse Embryonic Stem Cells)
Specification Day 4 differentiated Mouse Embryonic Stem Cells
Fixative Formaldehyde
Permeabilization Yes - PBS-0.3% Tween
Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Dr. Vladimir Mazurov

Verified customer

提交于 Aug 25 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (E6.5 embryo)
Specification E6.5 embryo
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric Acid
Permeabilization Yes - Tween
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Ms. Mary Trask

Verified customer

提交于 Jan 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"