MitoTox™ Complex V OXPHOS Activity Assay试剂盒(ab109907)

概述

  • 产品名称MitoTox™ Complex V OXPHOS Activity Assay试剂盒
    参阅全部 Complex V 试剂盒
  • 检测方法Colorimetric
  • 样品类型
    Inhibitor compounds
  • 检测类型Direct
  • 检测时间
    4h 00m
  • 种属反应性
    与反应: Rat, Cow, Human
    不与反应: Mouse
  • 产品概述

    MitoTox™ Complex V OXPHOS Activity Assay Kit (ab109907) is designed for testing the direct inhibitory effect of compounds on Complex V activity in only 4 hours. Complex V extracted from the provided bovine heart mitochondria (a rich source of Complex V) is immunocaptured by specific antibodies on the plate. Complex V activity can be observed as decrease in absorbance at OD 340 nm. The intra-assay and inter-assay variation of this assay are both < 10%.


    Inhibitory effects of compounds on Complex I activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC50) format, where two compounds known to affect Complex V activity can be tested at 11 different data points in triplicate.


    Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases. 

  • 说明

    Store Phospholipids, Bovine heart mitochondria and Activity Buffer at -80°C. Store all other components at 4°C.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab109907 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent dilution.

    MitoTox™ Complex V OXPHOS Activity Assay Kit 图像

    • Dose response curve for aurovertin, a non-competitive inhibitor of Complex V.

    • Typical dose response curve for oligomycin. Assay was performed following the Dose Response Assay Procedure using oligomycin, a well known Complex V inhibitor. Oligomycin was prepared in DMSO to generate a 10 mM stock. Starting with a 50 µM final concentration in well, 1:10 serial dilutions of oligomycin were generated.

    实验方案

    MitoTox™ Complex V OXPHOS Activity Assay Kit (ab109907)参考文献

    This product has been referenced in:
    • Xu Z  et al. Acute upregulation of neuronal mitochondrial type-1 cannabinoid receptor and it's role in metabolic defects and neuronal apoptosis after TBI. Mol Brain 9:75 (2016). Read more (PubMed: 27485212) »
    • Quan X  et al. Essential role of mitochondrial Ca2+ uniporter in the generation of mitochondrial pH gradient and metabolism-secretion coupling in insulin-releasing cells. J Biol Chem 290:4086-96 (2015). Read more (PubMed: 25548283) »

    See all 6 Publications for this product

    Product Wall

    Thank you for contacting us.

    Unfortunately none of our products measure the forward reaction. As far as I know there are no companies selling kits measuring the forward reaction of the ATP synthase I am afraid.

    I am sorry I could no...

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    The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
    ab109903, Complex I; reactivity = Human, mouse, rat, bovine
    http://www.abcam.com/mitotox%E2%84%A2-complex-i...

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    Thank you for contacting us.

    The bovine mitochondria is in Tris, sucrose, EDTA buffer pH 7.8


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Read More

    Product code: 109714 Inquiry: Hi Abcam, Do you know if this kit will immunocapture the ATP synthase enzyme from C. elegans? Unlikely unfortunately – this kit was tested positive with human, rat and cow. Mouse was negative. I think it is unl...

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    Unfortunately not. It comes with a sample (bovine) which cannot be substituted for mouse.

    The rate quoted in the booklet is mOD/min. I think this customer is quoting OD. Therefore 0.12/18 is 0.0067 OD/min = 6.7 mOD/min

    Changing the window analyzed to identify the steepest straight line slope is necessary and then to compare all sa...

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    Thank you for your enquiry.

    The negative control chosen for ab109907 MitoTox™ Complex V OXPHOS Activity Microplate Assaywould depend on whatyou want to accomplish and obtain from the experiment.

    Some people test the specific...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"