MitoTox™ Complex II OXPHOS Activity Assay试剂盒(ab109904)

概述

  • 产品名称MitoTox™ Complex II OXPHOS Activity Assay试剂盒
    参阅全部 Complex II 试剂盒
  • 检测方法Colorimetric
  • 检测类型Direct
  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 产品概述

    MitoTox™ Complex II OXPHOS Activity Assay Kit (ab109904) is designed for testing the direct inhibitory effect of compounds on Complex II activity in only 4 hours. Complex II extracted from the provided bovine heart mitochondria (a rich source of Complex II) is immunocaptured by specific antibodies on the plate. Complex II activity can be observed as decrease in absorbance at OD 600 nm. The intra-assay and inter-assay variation of this assay are both <15%.


    Inhibitory effects of compounds on Complex II activity can be tested in two different ways: 1. Screening format, where up to 23 compounds can be tested at a single concentration in triplicate; 2. Dose response (IC50) format, where two compounds known to affect Complex II activity can be tested at 11 different data points in triplicate.


    Testing for mitochondrial function has become a key aspect of drug discovery. Mitochondria can be affected by drug treatment, resulting into cardio- and hepatotoxic side effects that can lead to drug withdrawal from the market. Therefore, there is increasing emphasis on testing the impact on mitochondria early on in the drug development process to reduce failure rates during preclinical and clinical phases. 


     


     


     

  • 说明

    Please store Succinate, Ubiquinone 2, Bovine Heart Mitochondria, DCPIP at -80°C and all other components at 4°C.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab109904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex II OXPHOS Activity Assay Kit 图像

  • Typical dose response curve for TTFA (2-thenoyltrifluoracetone). Assay was performed following the Dose Response Assay Procedure using TTFA, a well known Complex II inhibitor. TTFA was prepared in DMSO to generate a 100 mM stock. Starting with a 500 µM final concentration in well, 1:2 serial dilutions of TTFA were generated.

实验方案

MitoTox™ Complex II OXPHOS Activity Assay Kit (ab109904)参考文献

This product has been referenced in:
  • Landry GM  et al. Diglycolic acid inhibits succinate dehydrogenase activity in human proximal tubule cells leading to mitochondrial dysfunction and cell death. Toxicol Lett 221:176-84 (2013). Read more (PubMed: 23827505) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine
http://www.abcam.com/mitotox%E2%84%A2-complex-i...

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ab109904 measures solely complex II, whereas ab109905 measures complexes II+III.
We do not offer an immunocapture activity assay for Complex III only. The reason is that we have not generated an antibody capable of immunocapturing the native enz...

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Thank you for contacting us.

The 10x detergent in the kit is 10% Lauryl maltoside, which can also be purchased separately as ab109857.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any...

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Thank you for your call and for your patience while I've been in touch with the lab about ab109904.

My contact at the lab gave the following reply: "Yes, a pH of 2 will destroy the assay. You will need to bring the pH to neutral ( 7 &nda...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"