MitoTox™ Complex V OXPHOS Activity Microplate Assay (ab109907)

概述

  • 产品名称MitoTox™ Complex V OXPHOS Activity Microplate Assay
    参阅全部 Complex V 试剂盒
  • 检测方法Colorimetric
  • 检测类型Direct
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Rat, Cow, Human
    不与反应: Mouse
  • 产品概述

    The MitoTox™ Complex V OXPHOS Activity Microplate Assay is designed for testing the direct inhibitory effect of compounds on Complex V activity using enzyme immunocaptured from bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex V. The phospholipids provided in this kit are essential for Complex V activity.

  • 说明

    Pre-coated 96-well microplate, Mito buffer, Wash Buffer and detergent should be stored at 4°C.Phopholipids should be stored at -20°C. Bovine heart mitochondria and Activity buffer should be stored at -80°C. Reagent reservoirs should be stored at room temperature.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

    应用

    Our Abpromise guarantee covers the use of ab109907 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent dilution.

    MitoTox™ Complex V OXPHOS Activity Microplate Assay 图像

    实验方案

    MitoTox™ Complex V OXPHOS Activity Microplate Assay (ab109907)参考文献

    This product has been referenced in:
    • Xu Z  et al. Acute upregulation of neuronal mitochondrial type-1 cannabinoid receptor and it's role in metabolic defects and neuronal apoptosis after TBI. Mol Brain 9:75 (2016). Read more (PubMed: 27485212) »
    • Chin RM  et al. The metabolite a-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature 509:397-401 (2014). Read more (PubMed: 24828042) »

    See all 5 Publications for this product

    Product Wall

    The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
    ab109903, Complex I; reactivity = Human, mouse, rat, bovine
    http://www.abcam.com/mitotox%E2%84%A2-complex-i...

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    Thank you for contacting us.

    The bovine mitochondria is in Tris, sucrose, EDTA buffer pH 7.8


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
    Read More

    Product code: 109714 Inquiry: Hi Abcam, Do you know if this kit will immunocapture the ATP synthase enzyme from C. elegans? Unlikely unfortunately – this kit was tested positive with human, rat and cow. Mouse was negative. I think it is unl...

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    Unfortunately not. It comes with a sample (bovine) which cannot be substituted for mouse.

    The rate quoted in the booklet is mOD/min. I think this customer is quoting OD. Therefore 0.12/18 is 0.0067 OD/min = 6.7 mOD/min

    Changing the window analyzed to identify the steepest straight line slope is necessary and then to compare all sa...

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    Thank you for contacting us.

    Unfortunately none of our products measure the forward reaction. As far as I know there are no companies selling kits measuring the forward reaction of the ATP synthase I am afraid.

    I am sorry I could no...

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    Thank you for your enquiry.

    The negative control chosen for ab109907 MitoTox™ Complex V OXPHOS Activity Microplate Assaywould depend on whatyou want to accomplish and obtain from the experiment.

    Some people test the specific...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"