MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906)

概述

  • 产品名称MitoTox™ Complex IV OXPHOS Activity Microplate Assay
    参阅全部 Complex IV 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 test
  • 检测类型Direct
  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Cow, Human
  • 产品概述

    ab109906 MitoTox™ OXPHOS Complex IV Activity Kit (MTOX4) is designed for screening the direct inhibitory effect of compounds on Complex IV activity and does this using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex IV.

  • 说明

    Store at -80°C: Bovine heart mitochondria

    Store at -20°C: Reagent C (store at -80°C for long term storage)

    Store at 4°C: Pre-coated 96-well plate, Blocking Buffer, Mito Buffer, Detergent

    Store at room temperature or 4°C: Blocking powder and reagent reservoirs

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab109906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex IV OXPHOS Activity Microplate Assay 图像

实验方案

MitoTox™ Complex IV OXPHOS Activity Microplate Assay (ab109906)参考文献

This product has been referenced in:
  • Chin RM  et al. The metabolite a-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature 509:397-401 (2014). Read more (PubMed: 24828042) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine
http://www.abcam.com/mitotox%E2%84%A2-complex-i...

Read More

This is because the plate requires fresh blocking to prevent non specific binding of the enzyme to the plate. We believe that this occurs because CIV is a highly hydrophobic enzyme.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"