MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)

概述

  • 产品名称MitoTox™ Complex II OXPHOS Activity Microplate Assay
    参阅全部 Complex II 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 检测类型Direct
  • 检测时间
    4h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Cow, Human
  • 产品概述

    MitoTox™ Complex II OXPHOS Activity Microplate Assay is designed for testing the direct inhibitory effect of compounds on Complex II activity using enzyme immunocaptured from Bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex II.

  • 说明

    Please store Succinate, Ubiquinone 2, BHM, DCPIP at -80°C and all other components at 4°C.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

应用

Our Abpromise guarantee covers the use of ab109904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex II OXPHOS Activity Microplate Assay 图像

实验方案

MitoTox™ Complex II OXPHOS Activity Microplate Assay (ab109904)参考文献

This product has been referenced in:
  • Landry GM  et al. Diglycolic acid inhibits succinate dehydrogenase activity in human proximal tubule cells leading to mitochondrial dysfunction and cell death. Toxicol Lett 221:176-84 (2013). Read more (PubMed: 23827505) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall

The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine
http://www.abcam.com/mitotox%E2%84%A2-complex-i...

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ab109904 measures solely complex II, whereas ab109905 measures complexes II+III.
We do not offer an immunocapture activity assay for Complex III only. The reason is that we have not generated an antibody capable of immunocapturing the native enz...

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Thank you for contacting us.

The 10x detergent in the kit is 10% Lauryl maltoside, which can also be purchased separately as ab109857.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any...

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Thank you for your call and for your patience while I've been in touch with the lab about ab109904.

My contact at the lab gave the following reply: "Yes, a pH of 2 will destroy the assay. You will need to bring the pH to neutral ( 7 &nda...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"