MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903)

概述

  • 产品名称MitoTox™ Complex I OXPHOS Activity Microplate Assay
    参阅全部 Complex I 试剂盒
  • 检测方法Colorimetric
  • 测试
    1 x 96 well plate
  • 检测类型Quantitative
  • 检测时间
    5h 00m
  • 实验步骤Multiple steps standard assay
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 产品概述

    MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903) is designed for screening the direct inhibitory effect of compounds on Complex I activity and does this using enzyme immunocaptured from bovine heart mitochondria. Bovine heart mitochondria are provided in this kit as they are a rich source of Complex I. The phospholipids provided in the kit are essential for rotenone-sensitive Complex I activity, rotenone being a well-known inhibitor of Complex I.

  • 说明

    Store Microplate, Wash buffer, Mito Buffer, and detergent at 4°C. Reagent reservoirs may be stored at RT. Store Phospholipids at -20°C. Store Bovine heart mitochondria, Ubiquinone and Activity Buffer at -80°C.

  • 经测试应用适用于: Functional Studiesmore details
  • 平台Microplate

性能

  • 别名
    • NADH dehydrogenase

应用

Our Abpromise guarantee covers the use of ab109903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent dilution.

MitoTox™ Complex I OXPHOS Activity Microplate Assay 图像

  • Dose Response Assay using Rotenone

实验方案

MitoTox™ Complex I OXPHOS Activity Microplate Assay (ab109903)参考文献

This product has been referenced in:
  • Kalghatgi S  et al. Bactericidal antibiotics induce mitochondrial dysfunction and oxidative damage in Mammalian cells. Sci Transl Med 5:192ra85 (2013). Read more (PubMed: 23825301) »
  • Lai K  et al. Integrated Compound Profiling Screens Identify the Mitochondrial Electron Transport Chain as the Molecular Target of the Natural Products Manassantin, Sesquicillin, and Arctigenin. ACS Chem Biol N/A:N/A (2012). Read more (PubMed: 23138533) »

See all 2 Publications for this product

Product Wall





The plate is one solid plate and not removable strips. Plate is best used at once as there is risk to unused wells getting wet, etc. We have not tried this and therefore cannot guarantee, but you could try putting a plate seal over...

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The kits that are the best suited for your experiment are listed below. I am having our datasheets team update the species reactivity.
ab109903, Complex I; reactivity = Human, mouse, rat, bovine
http://www.abcam.com/mitotox%E2%84%A2-complex-i...

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Thank you for contacting us.

I have checked with our MitoSciences lab and they sent the attached QC data for the plates used in that lot. Additionally, these kits are designed to be run with developmental compounds. On occasion, we have seen ...

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Les phospholipides peuvent en effet être le paramètre critique pour le bon fonctionnement du kit MTOX I.Ils ne sont pas stables si ils sont conservés à 4ºC et doivent être impérativement stockés &agra...

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Je suis ravi que les suggestions aient été utiles.

J'ai transmis votre question au laboratoire et vous contacterai de nouveau dès que j'aurai reçu une réponse.

Merci de votre réponse.

Le laboratoire vient de m'indiquer que la valeur de laDO initiale mesurée lors des tests de contrôle qualité était de 1 pour le lot GR78475-2.

Le problème rencontr&eac...

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Merci de nous avoir contactés.

La DO initiale (0.5) est en effet un peu faible et pourrait expliquer la valeur anormalementélevée de l'IC50.

Une des raisons expliquant la faible DO est l'age du kit et les condit...

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Thank you for your enquiry.

The solvent for the Ubiquinone 1 component is 100% ethanol.

Thank you for your enquiry. Please find the answers to your questions below.

1) Thank you for your feedback. We will take this into consideration with the production team.

2) Typically customers have used this kit in combination w...

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Thank you for your enquiry and your interest in our products. ab109905 is the only assay which is not an immunoassay. The supplied bovine sample can be replaced with a mitochondrial sample from any other species. This may require some optimization ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"