RabMAb

Anti-Mitofusin 2抗体[NIAR164] (ab124773)

概述

  • 产品名称Anti-Mitofusin 2抗体[NIAR164]
    参阅全部 Mitofusin 2 一抗
  • 描述
    兔单克隆抗体[NIAR164] to Mitofusin 2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to Human Mitofusin 2.
    Database link: O95140

  • 阳性对照
    • IHC-P: Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    This antibody was developed as part of a collaboration between the National Institutes of Health and the lab of Paritosh Ghosh.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Mitofusin 2 antibody (HRP) [NIAR164] (ab195543)

性能

应用

Our Abpromise guarantee covers the use of ab124773 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/300.

For unpurified, use 1/100 - 1/250.

靶标

  • 功能Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • 组织特异性Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • 疾病相关Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • 序列相似性Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • 翻译后修饰Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • 细胞定位Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Anti-Mitofusin 2 antibody [NIAR164] 图像

  • All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

    Lane 1 : mouse brain lysate
    Lane 2 : mouse kidney lysate
    Lane 3 : rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • All lanes : Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/5000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HEK293 cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence staining of HEK293 cells with purified ab124773 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124773 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • Immunohistochemical staining of paraffin embedded human kidney with purified ab124773 at a working dilution of 1/300. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Anti-Mitofusin 2 antibody [NIAR164] (ab124773) at 1/1000 dilution (unpurified) + Rat primary neurons cell lysate at 20 µg

    Secondary
    Anti-rabbit IgG HRP conjugate at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 86 kDa


    Exposure time : 30 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Unpurified ab124773, at 1/50, staining Mitofusin 2 in formalin fixed paraffin embedded Human kidney tissue using immunohistochemistry.

Anti-Mitofusin 2 antibody [NIAR164] (ab124773)参考文献

This product has been referenced in:
  • Kitaoka Y  et al. Combined effects of resistance training and calorie restriction on mitochondrial fusion and fission proteins in rat skeletal muscle. J Appl Physiol (1985) 121:806-810 (2016). Read more (PubMed: 27539498) »
  • Cherry AD  et al. Peroxisome proliferator-activated receptor ? co-activator 1-a as a critical co-activator of the murine hepatic oxidative stress response and mitochondrial biogenesis in Staphylococcus aureus sepsis. J Biol Chem 289:41-52 (2014). WB ; Mouse . Read more (PubMed: 24253037) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Primary Neurons)
Loading amount 20 µg
Specification Primary Neurons
Gel Running Conditions Reduced Denaturing (4-12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 May 15 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"