重组Anti-Mitofusin 2抗体[EPR19796] (ab205236)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19796] to Mitofusin 2
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Knockout validated
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
概述
-
产品名称
Anti-Mitofusin 2抗体[EPR19796]
参阅全部 Mitofusin 2 一抗 -
描述
兔单克隆抗体[EPR19796] to Mitofusin 2 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Human Mitofusin 2 recombinant protein fragment; Human fetal heart, fetal kidney and fetal liver lysates; HeLa and HEK-293 whole cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19796 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab205236于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/700.
|
|
WB |
1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
|
|
IP |
1/30.
|
|
ICC/IF |
1/250.
|
说明 |
---|
Flow Cyt (Intra)
1/700. |
WB
1/2000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa). |
IP
1/30. |
ICC/IF
1/250. |
靶标
-
功能
Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions. -
组织特异性
Ubiquitous; expressed at low level. Highly expressed in heart and kidney. -
疾病相关
Charcot-Marie-Tooth disease 2A2
Neuropathy, hereditary motor and sensory, 6A -
序列相似性
Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
Contains 1 dynamin-type G (guanine nucleotide-binding) domain. -
翻译后修饰
Phosphorylated by PINK1.
Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion. -
细胞定位
Mitochondrion outer membrane. Colocalizes with BAX during apoptosis. - Information by UniProt
-
数据库链接
- Entrez Gene: 9927 Human
- Omim: 608507 Human
- SwissProt: O95140 Human
- Unigene: 376681 Human
-
别名
- CMT2A antibody
- CMT2A2 antibody
- CPRP 1 antibody
see all
图片
-
All lanes : Anti-Mitofusin 2 antibody [EPR19796] (ab205236) at 1/2000 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : MFN2 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 86 kDaLanes 1 - 4: Merged signal (red and green). Green - ab205236 observed at 86 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab205236 was shown to recognize MFN2 (Mitofusin 2) in wild-type HEK-293 cells as signal was lost at the expected MW in MFN2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and MFN2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab205236 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-Mitofusin 2 antibody [EPR19796] (ab205236) at 1/5000 dilution
Lane 1 : Human Mitofusin 1 recombinant protein fragment
Lane 2 : Human Mitofusin 2 recombinant protein fragment
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 86 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human Mitofusin 1 recombinant protein fragment contains aa130-485 with a His-Tag®. Human Mitofusin 2 recombinant protein fragment contains aa151-506 with a His-Tag®.
-
All lanes : Anti-Mitofusin 2 antibody [EPR19796] (ab205236) at 1/5000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 15 seconds; Lane 2 and 3: 30 seconds.
The expression profile is consistent with the literature (PMID 14561718; PMID 25574749).
-
All lanes : Anti-Mitofusin 2 antibody [EPR19796] (ab205236) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 86 kDa
Observed band size: 86 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 with ab205236 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing mitochondrial staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). COX IV is detected with ab33985 (Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker), at 1/200 dilution, followed by secondary detection using ab150120 Alexa Fluor® 594 Goat anti-Mouse (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 with ab205236 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/200 dilution, followed by secondary detection using ab150120 Alexa Fluor® 594 Goat anti-Mouse (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Mitofusin 2 with ab205236 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
-
Mitofusin 2 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab205236 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab205236 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10 μg (Input).
Lane 2: ab205236 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab205236 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (8)
ab205236 被引用在 8 文献中.
- Mengus C et al. VCP/p97 cofactor UBXN1/SAKS1 regulates mitophagy by modulating MFN2 removal from mitochondria. Autophagy 18:171-190 (2022). PubMed: 33966597
- Li Y et al. Genistein improves mitochondrial function and inflammatory in rats with diabetic nephropathy via inhibiting MAPK/NF-κB pathway. Acta Cir Bras 37:e370601 (2022). PubMed: 35976278
- Kshirsagar S et al. A Combination Therapy of Urolithin A+EGCG Has Stronger Protective Effects than Single Drug Urolithin A in a Humanized Amyloid Beta Knockin Mice for Late-Onset Alzheimer's Disease. Cells 11:N/A (2022). PubMed: 36078067
- Araya LE et al. Deorphanizing Caspase-3 and Caspase-9 Substrates In and Out of Apoptosis with Deep Substrate Profiling. ACS Chem Biol 16:2280-2296 (2021). PubMed: 34553588
- Zhang W et al. GPR43 regulation of mitochondrial damage to alleviate inflammatory reaction in sepsis. Aging (Albany NY) 13:22588-22610 (2021). PubMed: 34584017
- Pang Y et al. HIGD-1B inhibits hypoxia-induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytes. Mol Med Rep 24:N/A (2021). PubMed: 34080026
- Li C et al. STING1 Promotes Ferroptosis Through MFN1/2-Dependent Mitochondrial Fusion. Front Cell Dev Biol 9:698679 (2021). PubMed: 34195205
- Zhuan B et al. Hypoxia induces pulmonary artery smooth muscle dysfunction through mitochondrial fragmentation-mediated endoplasmic reticulum stress. Aging (Albany NY) 12:23684-23697 (2020). PubMed: 33221740