Anti-Mitofusin 2抗体(ab50843)

概述

  • 产品名称Anti-Mitofusin 2抗体
    参阅全部 Mitofusin 2 一抗
  • 描述
    兔多克隆抗体to Mitofusin 2
  • 经测试应用适用于: ICC/IF, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Cynomolgus Monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Mitofusin 2 aa 557-576 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    C-LGPKNSRRALMGYNDQVQRP

  • 阳性对照
    • Mouse brain mitochondrial extract; rat brain mitochondrial extract; HeLa cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab50843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/200.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 86 kDa (predicted molecular weight: 84 kDa).
IP Use at 5-10 µg/mg of lysate. (using HeLa cell lysate)

靶标

  • 功能Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN2 acts independently of the cytoskeleton. It therefore plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Overexpression induces the formation of mitochondrial networks. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PARK2 recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions.
  • 组织特异性Ubiquitous; expressed at low level. Highly expressed in heart and kidney.
  • 疾病相关Charcot-Marie-Tooth disease 2A2
    Neuropathy, hereditary motor and sensory, 6A
  • 序列相似性Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
    Contains 1 dynamin-type G (guanine nucleotide-binding) domain.
  • 翻译后修饰Phosphorylated by PINK1.
    Ubiquitinated by non-degradative ubiquitin by PARK2, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion.
  • 细胞定位Mitochondrion outer membrane. Colocalizes with BAX during apoptosis.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMT2A antibody
    • CMT2A2 antibody
    • CPRP 1 antibody
    • CPRP1 antibody
    • EC 3.6.5.- antibody
    • Fzo antibody
    • HSG antibody
    • hyperplasia suppressor gene antibody
    • Hypertension related protein 1 antibody
    • KIAA0214 antibody
    • MARF antibody
    • MFN 2 antibody
    • Mfn2 antibody
    • MFN2_HUMAN antibody
    • Mitochondrial assembly regulatory factor antibody
    • Mitofusin-2 antibody
    • Mitofusin2 antibody
    • Transmembrane GTPase MFN2 antibody
    see all

Anti-Mitofusin 2 antibody 图像

  • All lanes : Anti-Mitofusin 2 antibody (ab50843) at 0.25 µg/ml

    Lane 1 : Rat brain mitochondria
    Lane 2 : Mouse brain mitochondria

    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate and a chemiluminescent substrate.

    Predicted band size : 84 kDa
    Observed band size : 86 kDa (why is the actual band size different from the predicted?)
  • ab50843 staining Mitofusin 2 in murine cardiac myocyte cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using 1% Triton X-100, blocked with 10% serum for 1 hour at room temperature, then incubated with ab50843 at a 1/200 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

Anti-Mitofusin 2 antibody (ab50843)参考文献

ab50843 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (mouse heart)
Total protein in input 200 µg
Specification mouse heart
Immuno-precipitation step Protein G
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提交于 Feb 08 2013

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Application Western blot
Sample Mouse Tissue lysate - whole (mouse heart)
Loading amount 20 µg
Specification mouse heart
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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提交于 Oct 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse cardiac myocyte)
Specification mouse cardiac myocyte
Fixative Formaldehyde
Permeabilization Yes - 1% triton x100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
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提交于 Sep 29 2011

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Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Loading amount 20 µg
Specification Mouse Embryonic Fibroblasts
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293T)
Loading amount 20 µg
Specification HEK 293T
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Dec 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"