概述

  • 产品名称
    Anti-Mitofusin 1抗体
    参阅全部 Mitofusin 1 一抗
  • 描述
    兔多克隆抗体to Mitofusin 1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 683-732 (QVDITQKQLE EEIARLPKEI DQLEKIQNNS KLLRNKAVQL ENELENFTKQ) of Human Mitofusin 1 (NP_284941).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照
    • Human placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab104274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 84 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • 组织特异性
    Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • 序列相似性
    Belongs to the mitofusin family.
  • 翻译后修饰
    Ubiquitinated by MARCH5.
  • 细胞定位
    Cytoplasm and Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Fzo homolog antibody
    • Hfzo1 antibody
    • Hfzo2 antibody
    • MFN 1 antibody
    • Mfn1 antibody
    • MFN1_HUMAN antibody
    • Mitochondrial transmembrane GTPase Fzo 1 antibody
    • Mitochondrial transmembrane GTPase FZO 2 antibody
    • Mitochondrial transmembrane GTPase FZO1B antibody
    • Mitofusin 1 antibody
    • Mitofusin-1 antibody
    • Mitofusin1 antibody
    • MS996 antibody
    • Putative transmembrane GTPase antibody
    • Transmembrane GTPase MFN1 antibody
    see all

图片

  • Western blot - Mitofusin 1 antibody (ab104274)
    Western blot - Mitofusin 1 antibody (ab104274)
    Anti-Mitofusin 1 antibody (ab104274) at 1 µg/ml + Human placenta lysate at 10 µg

    Predicted band size: 84 kDa

  • Western blot - Anti-Mitofusin 1 antibody (ab104274)
    Western blot - Anti-Mitofusin 1 antibody (ab104274)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Mitofusin 1 antibody (ab104274) at 1/1000 dilution (+ 5% non-fat milk for 16 hours at 4°C )

    Lanes 1-2 : Whole tissue lysate of mouse heart (wild type)
    Lanes 3-4 : Whole tissue lysate of mouse heart (Knock-out)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 84 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 minute


    Blocking Step: 5% Milk for 1 hour at room temperature

文献

This product has been referenced in:

See all 10 Publications for this product

发表研究结果有使用 ab104274？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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5 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-Mitofusin 1 antibody

 Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Prosate cancer cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Prosate cancer cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Abcam user community

Verified customer

提交于 Dec 11 2017

Western blot abreview for Anti-Mitofusin 1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - other (Mitochondrial Isolate)
Gel Running Conditions
Reduced Denaturing (4-12% gel)
Loading amount
20 µg
Specification
Mitochondrial Isolate
Blocking step
Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
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Username

Abcam user community

Verified customer

提交于 Nov 22 2016

Totally unacceptable policy. The responsible scientific journals require a complete description of the immunogen when claiming results from a particular antibody. I will in future purchase antibodies from a more ethical company.

I believe there has been a misunderstanding here.

We try to provide all the information which we have regarding any product we carry and provide the most thorough data we can on our datasheets as you have undoubtably seen when using our Mito...

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Previously we used your chicken polyclonal to MFN1 (ab60939) to good effect on dog tissue for WB and IHC-P(Fyfe JC et al Neurogentics 2011;12:223-232, (fig 1 and 2). Now when we need it again, I find you do not have it. Since we are working in dog, I n...

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Thank you very much for your interest in our Mitofusin 1 antibodies.

Unfortunately the exact sequences of many of the immunogens used to create our proprietary. I have BLAST-ed the human and mouse Mitofusin 1 sequences and found that the ide...

Read More

Western blot abreview for Anti-Mitofusin 1 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse heart)
Loading amount
20 µg
Specification
mouse heart
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 Oct 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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