This antibody gave a positive signal in both HepG2 and U87MG whole cell lysates, as well as in the following tissue lysates: Human Liver; Human Heart; Human Brain; Human testis.
This antibody gave a positive result in IF in the following methanol fixed cell lines: MCF-7
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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Western blot - Anti-Mitofilin antibody (ab109424)
All lanes : Anti-Mitofilin antibody (ab109424) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889) Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : Human heart tissue lysate - total protein (ab29431) Lane 4 : Human brain tissue lysate - total protein (ab29466) Lane 5 : Human testis tissue lysate - total protein (ab30257) Lane 6 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
ICC/IF image of ab109424 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab109424 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.