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Synthetic peptide corresponding to N-terminal residues of mouse Mitoferrin-1
This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
Our Abpromise guarantee covers the use of ab56134 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.|
|ICC/IF||Use at an assay dependent concentration.|
ab56134 staining cultured rat iron treated astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde in 0.3% TritonX in 0.1% PBS for 5 minutes and blocked with 10% donkey serum in 0.3% TritonX in 0.1% PBS for 30 minutes at 24°C. The cultured cells were then stained with ab561340 at 1/200 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab56134 has not yet been referenced specifically in any publications.