Anti-Mitoferrin1抗体(ab56134)

概述

  • 产品名称
    Anti-Mitoferrin1抗体
    参阅全部 Mitoferrin1 一抗
  • 描述
    兔多克隆抗体to Mitoferrin1
  • 经测试应用
    适用于: ICC, ELISA, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Recombinant fragment
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to N-terminal residues of mouse Mitoferrin-1

  • 常规说明

    This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.

性能

应用

Our Abpromise guarantee covers the use of ab56134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC 1/200.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.

靶标

  • 相关性
    Mitoferrin1, SLC25A37, belongs to the mitochondrial carrier family and contains 3 Solcar repeats. It is a mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells and plays an essential role in heme biosynthesis.
  • 细胞定位
    Mitochondrial inner membrane; Multi-pass membrane protein
  • 数据库链接
  • 别名
    • HT015 antibody
    • MFRN antibody
    • Mitochondrial iron transporter 1 antibody
    • Mitochondrial solute carrier protein antibody
    • Mitoferrin 1 antibody
    • MSC antibody
    • MSCP antibody
    • predicted protein of HQ2217 antibody
    • PRO1278 antibody
    • PRO1584 antibody
    • PRO2217 antibody
    • Solute carrier family 25 member 37 antibody
    see all

图片

  • ab56134 staining cultured rat iron treated astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde in 0.3% TritonX in 0.1% PBS for 5 minutes and blocked with 10% donkey serum in 0.3% TritonX in 0.1% PBS for 30 minutes at 24°C. The cultured cells were then stained with ab561340 at 1/200 in 0.3% TritonX with 0.1x PBS and 10% donkey serum for 24h at 4°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Hoechst was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

文献

ab56134 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (DRG neurons)
Specification
DRG neurons
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Mar 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Iron treated astrocytes)
Specification
Iron treated astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

提交于 Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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