Anti-Mitochondrial Pyruvate dehydrogenase kinase 1抗体(ab90444)

概述

  • 产品名称Anti-Mitochondrial Pyruvate dehydrogenase kinase 1抗体
    参阅全部 Mitochondrial Pyruvate dehydrogenase kinase 1 一抗
  • 描述
    兔多克隆抗体to Mitochondrial Pyruvate dehydrogenase kinase 1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Dog, Human, Pig, Monkey
  • 免疫原

    Synthetic peptide derived from the sequence of human PDK1, conjugated to KLH

  • 阳性对照
    • HeLa, Rat brain, Mouse brain, Vero, ESK-4, RK-13 or MDCK cytoplasmic or membrane lysate Human hepatoma tissue IHC-P: FFPE human colon tissue sections.

应用

Our Abpromise guarantee covers the use of ab90444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 49 kDa.
IHC-P Use at an assay dependent concentration.

靶标

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody 图像

  • IHC image of Mitochondrial Pyruvate dehydrogenase kinase 1 staining in human colon formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90444, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab90444) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Vero cell lysate
    Lane 5 : ESK-4 cell lysate
    Lane 6 : RK-13 cell lysate
    Lane 7 : MDCK cell lysate


    Predicted band size : 49 kDa

Anti-Mitochondrial Pyruvate dehydrogenase kinase 1 antibody (ab90444)参考文献

ab90444 has not yet been referenced specifically in any publications.

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